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cDNA Synthesis Kit
Order no.: BIO-65025
(Pack Size: 30rxns)
- Features
- Generate high quality cDNA for any downstream application
- Highly suited to low abundance Total RNA ≥100pg
- Convenient, reliable, cost-effective
- Applications
- 1st strand cDNA synthesis
- Construction of cDNA libraries
- 2-step RT PCR assays
- Generation of probes for hybridization
- Gene cloning
- Description
The cDNA Synthesis Kit contains all necessary components to generate
cDNA from an RNA template. The generated cDNA is suitable for PCR with
gene-specific primers or for other downstream applications. The kit
contains a reverse transcriptase and is suitable for first strand cDNA
synthesis, cDNA library construction, and the production of templates
for RT-PCR amplifications.
The cDNA Synthesis Kit is optimized for RT reactions using a wide range
of total RNA amounts (100pg-2µg), such that long and low abundance cDNAs
can be detected by amplification after cDNA synthesis. The kit contains
oligo
(dT)18 and random hexamer
primers together with control RNA template. The kit components are
fully optimized to generate maximum yields of full-length cDNA. The dNTPs
included in the kit are manufactured by Bioline and are 99% pure.
- Kit Components
- Storage Conditions
The cDNA Synthesis Kit can be stored for 6 months at -20°C.
- Product Citations
1. Zhou, C.C., et al. Circulation Research 100(1), 88-95
(2007).
CHF 296.00
Price without VAT or delivery
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