Innovative instrumentation for life sciences

 

 

Already fully equipped for real-time amplification, end-point analysis, high-resolution melt analysis, and nucleic acid concentration measurements.

 

- Detect all available real-time chemistries for extreme compatibility

- Up to six channels for more flexibility in multiplex application

- High-Resolution melt function to discriminate single base mutation

- High speed runs to increase productivity

- 100-well rotor to support 96 samples and additional controls (More information)

Download brochure (2Mo PDF)

INFORMATIONS :

Why a rotor?

Web site factory Corbett Research

Publications

Kit Validtion OTV ™

Request documentation / references / offer

 

  

Rotor-Gene 6000

 

 

The most versatile real-time instrument ever made

 

 
   High Resolution Melt  (More information)    
HRM is a new application that can greatly extend the utility of traditional DNA melting analysis. HRM is made possible by the combination of more advanced instrument design and new dye technology.

The Rotor-Gene 6000 has been engineered for HRM. It incorporates a specially tuned high-intensity optical channel, high-speed data capture (up to 1000 data collection points per °C transition), extreme thermal resolution (0.02°C) and dedicated analysis software. New “saturation” dyes such as SYTO 9 and LC Green provide the best results since they can be used at higher concentrations to provide increased resolution over traditional dyes (e.g. SYBR Green 1).

HRM characterizes nucleic acid samples based on their association (annealing) or disassociation (melting) behavior. Sequences can be discriminated by length, GC content and complementarity. Even single base changes such as SNPs (single nucleotide polymorphisms) can be readily identified. An example of SNP genotyping by HRM is shown opposite.

 

    

 

 
HRM Applications

Many applications are possible for HRM analysis. Some of those currently under investigation include:

1. Mutation discovery/Screening/Scanning

2. SNP genotyping/Allelic discrimination
    - Identify candidate genes 
    - Association (case/control) studies 
    - Prevalence (populations or groups)
    - Loss of heterozygosity 
    - DNA fingerprinting 
    - Haplotype blocks

3. Predicative testing
     - Penetrance/Linkage studies

4. Species identification

5. Epigenetics/DNA methylation

6. Microsatellite analysis 
    (tandem repeats)
     - linkage
     - fingerprinting
     - instability
     - loss of hetrozygosity

 

  

SNP Genotyping by High Resolution Melt (HRM)
Discrimination of human ACTN3 (R577X) SNP genotypes (C to T substitution) using SYTO® 9 intercalation dye (no probes). Homozygous wild type, mutation and heterozygote samples are shown on a standard normalized melt curve (A) and a difference plot normalized to heterozygous samples (B). Genotypes are automatically assigned by the software. Fragment pre-amplified using a 40 cycle fast protocol (<40 min).

 

INFORMATIONS :

Why a rotor?

Web site factory Corbett Research

Publications

Kit Validtion OTV ™

Request documentation / references / offer

 

BIOLABO Scientific Instruments - Z.I. Pra-dePlan 35 - 1618 Châtel-St-Denis - Switzerland

Tel : 0041 (0)21 948 7715  -  Fax : 0041 (0)21 948 7804 - info@biolabo.ch

 

Copyright © 1994 BIOLABO Scientific Instruments SA
Last modification : 20-08-2008 16:56